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4. | | SAYD, R. M.; AMABILE, R. F.; FALEIRO, F. G.; MONTALVÃO, A. P. L.; COELHO, M. C. Agronomic characterization of high-yielding irrigated barley accessions in the Cerrado. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 52, n. 2, p. 84-94, fev. 2017. Título em português: Caracterização agronômica de acessos de cevada de alta produtividade sob irrigação no Cerrado. Biblioteca(s): Embrapa Cerrados; Embrapa Unidades Centrais. |
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5. | | SAYD, R. M.; AMABILE, R. F.; CARVALHO, C. G. P. de; FALEIRO, F. G. Avaliação temporal de genótipos de girassol no cerrado do Distrito Federal em safrinha. In.: REUNIÃO NACIONAL DE PESQUISA DE GIRASSOL, 20.; SIMPÓSIO NACIONAL SOBRE A CULTURA DO GIRASSOL, 8., 2013, Cuiabá. Anais... Brasília, DF: Embrapa, 2013. p. 135-138. Biblioteca(s): Embrapa Cerrados. |
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6. | | SAYD, R. M.; AMABILE, R. F.; CARVALHO, C. G. P. de; FALEIRO, F. G. Avaliação temporal de genótipos de girassol no cerrado do Distrito Federal em safrinha. In: REUNIÃO NACIONAL DE PESQUISA DE GIRASSOL, 20.; SIMPÓSIO NACIONAL SOBRE A CULTURA DO GIRASSOL, 8., 2013, Cuiabá. Anais... Brasília, DF: Embrapa, 2013. p. 135-138. Biblioteca(s): Embrapa Soja. |
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8. | | SAYD, R. M.; AMABILE, R. F.; FALEIRO, F. G.; BELLON, G. Genetic variability of hull-less barley accessions based on molecular and quantitative data. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 50, n. 2, p. 160-167, fev. 2015. Biblioteca(s): Embrapa Cerrados; Embrapa Unidades Centrais. |
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11. | | SAYD, R. M.; AMABILE, R. F.; FALEIRO, F. G.; PEIXOTO, J. R.; MONTALVÃO, A. P. L. Seleção de acessos de cevada de alta produtividade em sistema de produção irrigado no Cerrado. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CERRADOS, 2015, Planaltina, DF. Jovens Talentos 2015: resumos. Planaltina, DF: Embrapa Cerrados, 2015. (Embrapa Cerrados. Documentos, 328). p. 38. Biblioteca(s): Embrapa Cerrados. |
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12. | | SANTIN, M. R.; COELHO, M. C.; SAYD, R. M; PEIXOTO, J. R.; AMABILE, R. F. Yield, maturation cycle, and estimates of genetic parameters of Robusta coffee genotypes under irrigation in the Cerrado. Crop Breeding and Applied Biotechnology, v. 19, n. 4, 2019. p. 387-394 Biblioteca(s): Embrapa Cerrados. |
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13. | | THOMÉ, R. D.; AMABILE, R. F.; MALAQUIAS, J. V.; SOUZA, N. O. S.; SAYD, R. M.; LOURES, L. M. R. Adaptabilidade e estabilidade de genótipos de cevada irrigada no Cerrado do Brasil Central In: SIMPÓSIO DE MELHORAMENTO DE PLANTAS, 3., 2020. O melhoramento sem fronteiras. Brasília, DF: SBMP, 2020. Biblioteca(s): Embrapa Cerrados. |
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14. | | AMABILE, R. F.; CARVALHO, C. G. P. de; SAYD, R. M.; MONTEIRO, V. A.; RIBEIRO JUNIOR, W. Q. Avaliação de genótipos de girassol em safrinha no cerrado do Distrito Federal em 2011 em ensaio de segundo ano. In: REUNIÃO NACIONAL DE PESQUISA DE GIRASSOL, 19.; SIMPÓSIO NACIONAL SOBRE A CULTURA DO GIRASSOL, 7., 2011, Aracaju. Anais... Londrina: Embrapa Soja; Aracaju: Embrapa Tabuleiros Costeiros, 2011. 1 CD-ROM. p. 354-356. Biblioteca(s): Embrapa Cerrados. |
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16. | | OLIVEIRA, S. O. D. de; SAYD, R. M.; SILVA, R. de C. S.; SCHERWINSKI PEREIRA, J. E. Interação de tipos de carboidratos adicionados ao meio de cultura na conservação In vitro de aspargo (Asparagus officinalis). SIMPÓSIO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2008, Brasília, DF. Anais... Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia: Fundação de Apoio à Pesquisa Científica e Tecnológica - FUNCREDI, 2008. p.363. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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17. | | AMABILE, R. F.; FALEIRO, F. G.; CAPETTINI, F.; SAYD, R. M.; PEIXOTO, J. R.; GUERCIA, R. F. Characterization and genetic variability of barley accessions (Hordeum vulgare L.) irrigated in the savannas based on malting quality traits. Journal of the Institute of Brewing, v. 120, n. 4, p. 404-414, 2014. Biblioteca(s): Embrapa Cerrados. |
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18. | | AMABILE, R. F.; CARVALHO, C. G. P. de; SAYD, R. M.; MONTEIRO, V. A.; RIBEIRO JUNIOR, W. Q. Comportamento de genótipos de girassol em safrinha no cerrado do Distrito Federal em 2011 em ensaio de primeiro ano. In: REUNIÃO NACIONAL DE PESQUISA DE GIRASSOL, 19.; SIMPÓSIO NACIONAL SOBRE A CULTURA DO GIRASSOL, 7., 2011, Aracaju. Anais... Londrina: Embrapa Soja; Aracaju: Embrapa Tabuleiros Costeiros, 2011. 1 CD-ROM. p. 351-353. Biblioteca(s): Embrapa Cerrados. |
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Registros recuperados : 55 | |
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Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
22/03/2017 |
Data da última atualização: |
29/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
OLIVEIRA, S. O. D. de; SAYD, R. M.; BALZON, T. A.; SCHERWINSKI PEREIRA, J. E. |
Afiliação: |
Sharrine Omari Domingues de Oliveira, Universidade Federal de Viçosa; Ricardo Meneses Sayd, UnB; Talita Aparecida Balzon, Universidade Federal do Amazonas; JONNY EVERSON SCHERWINSKI PEREIRA, Cenargen. |
Título: |
A new procedure for in vitro propagation of vanilla (Vanilla planifolia) using a double-phase culture system. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Scientia Horticulturae, v. 161, p. 204-209, 2013. |
Idioma: |
Inglês |
Conteúdo: |
We have developed an efficient and simplified method for shoot proliferation of vanilla (Vanilla planifolia), using a Double-Phase Culture System (DPS) (semi-solid medium with a layer of liquid medium on the top). In vitro proliferating shoot cultures were the source of nodal explants (approximately 1.0 cm in height and at least one axillary bud) used in all experiments. Three experiments were conducted: the first determined the influence of cultivation time (30, 60 and 90 days) and concentrations of 6-benzylaminopurine (BA) (0,1, 2 and 3 mg l−1) on in vitro multiplication of vanilla; the second evaluated the influence of conventional (semi-solid medium, or SS) and DPS systems on in vitro multiplication of vanilla. Finally, we evaluated the effect of indole-3-butyric acid (IBA) (0, 1, 2 and 3 mg l−1) concentrations on the rooting of shoots multiplied in vitro and on survival rates after acclimatization. BA concentration in the culture medium and DPS for shoot multiplication had significant effects on success. The multiplication rate, which reflects axillary shoot multiplication, was greatest when DPS with 1 mg l−1 BA was used, representing more than a 2.5-fold increase over the rates obtained with SS medium, after 90 days of cultivation. Shoots taken from DPS were rooted at a frequency of 100%, in full-strength Murashige and Skoog medium without IBA. The plantlets were then transferred to soil, and survived acclimatization with 100% success. The results indicate the establishment of a new procedure for vanilla micropropagation through DPS, with a high production of plantlets at the end of the process and great potential for agriculture application. In addition, the presented system is more economic than the single-phase during the subcultures. MenosWe have developed an efficient and simplified method for shoot proliferation of vanilla (Vanilla planifolia), using a Double-Phase Culture System (DPS) (semi-solid medium with a layer of liquid medium on the top). In vitro proliferating shoot cultures were the source of nodal explants (approximately 1.0 cm in height and at least one axillary bud) used in all experiments. Three experiments were conducted: the first determined the influence of cultivation time (30, 60 and 90 days) and concentrations of 6-benzylaminopurine (BA) (0,1, 2 and 3 mg l−1) on in vitro multiplication of vanilla; the second evaluated the influence of conventional (semi-solid medium, or SS) and DPS systems on in vitro multiplication of vanilla. Finally, we evaluated the effect of indole-3-butyric acid (IBA) (0, 1, 2 and 3 mg l−1) concentrations on the rooting of shoots multiplied in vitro and on survival rates after acclimatization. BA concentration in the culture medium and DPS for shoot multiplication had significant effects on success. The multiplication rate, which reflects axillary shoot multiplication, was greatest when DPS with 1 mg l−1 BA was used, representing more than a 2.5-fold increase over the rates obtained with SS medium, after 90 days of cultivation. Shoots taken from DPS were rooted at a frequency of 100%, in full-strength Murashige and Skoog medium without IBA. The plantlets were then transferred to soil, and survived acclimatization with 100% success. The results ind... Mostrar Tudo |
Palavras-Chave: |
Shoot proliferation. |
Thesagro: |
Micropropagação. |
Thesaurus NAL: |
micropropagation. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02408naa a2200193 a 4500 001 2067485 005 2023-03-29 008 2013 bl uuuu u00u1 u #d 100 1 $aOLIVEIRA, S. O. D. de 245 $aA new procedure for in vitro propagation of vanilla (Vanilla planifolia) using a double-phase culture system.$h[electronic resource] 260 $c2013 520 $aWe have developed an efficient and simplified method for shoot proliferation of vanilla (Vanilla planifolia), using a Double-Phase Culture System (DPS) (semi-solid medium with a layer of liquid medium on the top). In vitro proliferating shoot cultures were the source of nodal explants (approximately 1.0 cm in height and at least one axillary bud) used in all experiments. Three experiments were conducted: the first determined the influence of cultivation time (30, 60 and 90 days) and concentrations of 6-benzylaminopurine (BA) (0,1, 2 and 3 mg l−1) on in vitro multiplication of vanilla; the second evaluated the influence of conventional (semi-solid medium, or SS) and DPS systems on in vitro multiplication of vanilla. Finally, we evaluated the effect of indole-3-butyric acid (IBA) (0, 1, 2 and 3 mg l−1) concentrations on the rooting of shoots multiplied in vitro and on survival rates after acclimatization. BA concentration in the culture medium and DPS for shoot multiplication had significant effects on success. The multiplication rate, which reflects axillary shoot multiplication, was greatest when DPS with 1 mg l−1 BA was used, representing more than a 2.5-fold increase over the rates obtained with SS medium, after 90 days of cultivation. Shoots taken from DPS were rooted at a frequency of 100%, in full-strength Murashige and Skoog medium without IBA. The plantlets were then transferred to soil, and survived acclimatization with 100% success. The results indicate the establishment of a new procedure for vanilla micropropagation through DPS, with a high production of plantlets at the end of the process and great potential for agriculture application. In addition, the presented system is more economic than the single-phase during the subcultures. 650 $amicropropagation 650 $aMicropropagação 653 $aShoot proliferation 700 1 $aSAYD, R. M. 700 1 $aBALZON, T. A. 700 1 $aSCHERWINSKI PEREIRA, J. E. 773 $tScientia Horticulturae$gv. 161, p. 204-209, 2013.
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